Course Description

410.659 - Advanced Recombinant DNA Lab

This course is a continuation of 410.656 Recombinant DNA Laboratory, intended for those who have completed the introductory course or who have extensive molecular biology laboratory experience. This second course consists of a series of integrated laboratory exercises designed to give students hands-on experience with a variety of advanced recombinant DNA techniques. Exercises include molecular cloning, PCR optimization, site-directed mutagenesis, mutation detection, measuring gene expression by quantitative real-time PCR (qRT-PCR), and control of gene expression by RNA interference. Students will be introduced to high-throughput/high-content screening procedures, such as robotic liquid handling, microarray analysis, and utilization of bioinformatic technique Prerequisites: 410.601 Biochemistry, 410.602 Molecular Biology 410.656 Recombinant DNA Laboratory, or consent of program committee. S