Course Description

410.659 - Advanced Recombinant DNA Lab

This course is a continuation of Recombinant DNA Laboratory (410.656), intended for those who have completed the introductory course, or who have extensive molecular biology laboratory experience. This second course consists of a series of integrated laboratory exercises designed to give students hands-on experience with a variety of molecular techniques. Exercises include molecular cloning; PCR optimization; quantitative real time PCR; control of gene expression by RNA interference (RNAi), CRISPR and DNA sequencing. Students will be introduced microarray analysis, and utilization of bioinformatics pipelines. Prerequisites: 410.601 Biochemistry; 410.602 Molecular Biology; 410.656 Recombinant DNA Laboratory; or consent of program committee.