410.659 - Advanced Recombinant DNA Lab
This course is a continuation of Recombinant DNA Laboratory (410.656), intended for those who have completed the introductory course or who have extensive molecular biology laboratory experience. This second course consists of a series of integrated laboratory exercises designed to give students hands-on experience with a variety of advanced recombinant DNA techniques. Exercises include molecular cloning; PCR optimization; site directed mutagenesis; mutation detection; measuring gene expression by quantitative real time PCR (qRT-PCR); and control of gene expression by RNA interference (RNAi). Students will be introduced to high-throughput/high-content screening procedures, such as robotic liquid handling, microarray analysis, and utilization of bioinformatic techniques. Prerequisites: 410.601 Biochemistry; 410.602 Molecular Biology; 410.656 Recombinant DNA Laboratory; or consent of program committee.