Video Resources

Faculty and students of the JHU AAP Center for Biotechnology frequently contribute to academic publications around the world. Among those publications, some are video publications.

Here are some recent video publications, contributed by JHU AAP faculty and staff:

1. Pyrosequencing for Microbial Identification and Characterization, Patrick J. Cummings¹, Ray Ahmed², Jeffrey A. Durocher², Adam Jessen², Tamar Vardi², Kristina M. Obom¹

¹Center for Biotechnology Education, Krieger School of Arts and Sciences, Johns Hopkins University, ²Qiagen Sciences, Inc.

Summary: Pyrosequencing is a versatile technique that facilitates microbial genome sequencing that can be used to identify bacterial species, discriminate bacterial strains, and detect genetic mutations that confer resistance to anti-microbial agents. In this video, the procedure for microbial amplicon generation, amplicon pyrosequencing, and DNA sequence analysis will be demonstrated.

Date Published: 8/22/2013, Issue 78; doi: 10.3791/50405_

2. Operation of a Benchtop Bioreactor, Kristina M. Obom¹, Andrew Magno², Patrick J. Cummings¹

¹Center for Biotechnology Education, Krieger School of Arts and Sciences, Johns Hopkins University, ²ATR Biotech

Summary: Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.

Date Published: 9/12/2013, Issue 79; doi: 10.3791/50582